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() 2010 ()
,: 20 , 2 , 40 .. 1., A.DNA RNA B. DNA ATP C. T2 RNA D. DNA D ,.DNA ,RNA ,A ;DNA ATP C,H,O,N,P ,B .T2 DNA, DNA ,C . DNA ,,D . 2 , A. mRNA mRNA B. C. D., A .MRNA , mRNA , mRNA ,A . ,٧ܧ,B . mRNA ,C ., ,D . 3 129 . A. l29 l29 B.. C., D., B

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m,,., ,,A.,,B .,,C ., ,,D. 4 . 20 ,. A. B. C. DNA , D. C m.,, C .,,. 5.,,L. A. B. C. A . T , T , T , .A .,,B . , T ç T , T ,,C ,D . 6."",. A. B. C.Χئ D. D.

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B ,., ,A .,, ;,, 1/4,B . ,,C ,, ,D . 7.ց, A. B. C. D.~ C ., ,A .,,B . ,,,,C .,, , 20C ; 3035C,D . 8., A.ȧݧ B. C.Ԧ˧ D.˧ C .,, ,A ., ,,,B . ,,C ., ,D . 9.. A.

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B., C.,B D., B . .;,B ;,, .,, , B . 10.,. A., B. , C., D., D ,,,A , ,,B .C ,C .,, ,,D . 11.",",. A. B. C. D. C q., ,,.

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12 . A. B. C. D D . , . A ,,B . ,, ,C .,, .D . C ,,, .,, .,֧. 13 , A.ڧ B. b C.,a ˁ: D. D ., ,ܧڧ,A .b , ,,B . , a ,a ()() , () ,C ., ,,D . 14 ,,,

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A. B. C. D. B .,. ,B .C ., ,D . 15.֧٧, .ا

A. B. C. D. D ., ,,,, ,, D. 16.. .. A. B., C. D.. B .,B .

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17. , . . . A. B. c. D. A ., ,, , A 18.. A., B.. C. D., A .,,A ; ,,B ; ,C ;,,D . 19 .

A. C

8.

C.

D.

.(1) 0.3% ,(2) 20F,(4), 2,4-D , ,(3) C .

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20.,.G .g . g .G g g . g g .:Gg .g g . g g . A.Gg G g B. C. D.., D .,Gg,G g , ,A ; g g ,B ; g g g g ,C ;,,D . ,: 5 . 3 . 15 .. 3 .. 21., A. 92 B. C. D. 1/ 2 23 ABC .,, ,, 92 ,A ;, ,B ,,, C , 4 ,D 22.}.. A. B. c. D.


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BCD .,, , BCD. 23.. ,,

A. 10

6

mol/L

B. M , C. D. BD . 10-6 ,,A ; , M ,,B ;,, ,,C ,D . 24., A., B., C., D , ABCD . ͧ ,,, ,

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, ABCD. 25. 1 , 2 . A.B. 1 X CaCl2 , C. 2 D. 1 2 BCD .,A ; 1 ,B ; 2 ,C ; 1 2 ,D . ,: 9 .. 26.(7 ).: (1). _______;, . ().

(2) RNA

(3) CO2 (). .

(4),,

(5)߁,. (). , , , ,,, (1) () (2)

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(3) (4)() (5) ,, . (1),,;, ,() . (2)RNA mRNA,tRNA,rRNA ,, ,,,. (3),,,. (4),,, ,. (5),,, ,,,.

27. )ԧާڦ, l, 2 ަ (8 .:

(1) 1 Sma ,



. . .

(2), Sma, (3) DNA , Srna ,

(4) EcoR I , BamH Hind , DNA . .

(5),ڦ,

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(6)



.

(7) cDNA , , (1)0,2 (2) (3)Sma, DNA (4) DNA (5)DNA (6) (7) (1) DNA ,,. 1 , Sma,, DNA ,,. (2) ,Sma DNA G C, G C ,A T , Sma, (3) , 2 , DNA Sma , Sma, DNA (4) EcoR I,,, , BamH Hind ,, DNA ,. (5),, DNA . (6),. (7),, ,, ,. ,.

28. )( G1 ,S G2 )(M ). (6 12) DNA .:

(

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(1)(DNA ), S .,, . h M .

(2) M M , , .

(3), S , . h , S .

(4) 24 h,M l.9 h. , ("""").

(5),, (1)2.2 (2)M 122412 (3)7.4 (4) (5),;ڦ DNA , . (1) S ,,S G2 M , G2 , 2.2h (2), M , M , ,, M ., .

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12-24-12 (3), S ; S , G2,M,G1 S , 2.2+1.8+3.4=7.4h. S , 7.4h S (4), 1.8/(3.4+7.9+2.2+1.8)=1.8/15.3=11.2%; 1.9/24=0.08,, ,. (5),,, ,;.

29. ) (7 , , , (ڡ)

(1). (2). (3) II-4,II-6 .,.: (IV-1 IV-2). (IV-I),.(IV-2), . (1) (2) X (3)1/1296 1/36 0 , (1)"",,

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(2),, X (3) a, II8 ,I3 ,I4 Aa, II5 2/3Aa 1/3AAII6 , AA, III2 AA 2/3.*1/2=1/3, Aa 2/3, III1 2/3Aa 1/3 AA, 1/3*1/3*1/4=1/36, 1/36, 1/36*1/36=1/296, Y , III2 ,, IV-1 1/36*1=1/36. , IV2 0 : 1 : Y ()( ), X 2 (1), X . (2),. (3) , X . ,. 3 ,: (1),. (2),,. (3),,.

30.(8 )..ۧ. : (1) X 8 d., .,. (2) x .(). .. (3):. ... :. , x ,500 mL ,,

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,,.,(). : : 7000 mL . 8 d.. :. : 6 ... :. : 6 . .. (4)(3).. (1) (2) (3) X ;, x 3 150mL X , 3 150mL X 6 , 8d (1),,. (2),,. (3),¦ ,,,. ,,.

31.(6 ).,,., B . B ,.

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: (1).bc . (2),,, .. (3) (""""),. (1) (2) B . , cd

(3) , (1),,, ,, ,. (2),, B ,,,, ,. (3),, .

32.(7 ) DNA .: :, 2 . l0 g., 20, 20 24 h. DNA : :, l5 mL ,..

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. : 6 10mL , 20 mL 95% ,,. : 6 , 2 mol/L NaCl . DNA : 4 mL ., 5 min, ,.

,: (1) . .

(2)"", (3),.

1: 20,-20,DNA . 2: . .

I.:

(4),, DNA , DNA . DNA ,. DNA : 95% DNA . (1) DNA (2)DNA (3),, DNA ,DNA (4),, DNA ,,,, DNA ., DNA , ,,,DNA , ,DNA ,,, DNA , .

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,,,DNA , ,, DNA.

33.(8 ). A () B () ,. (1)., ,.A,B :

. , .

(2),ӧ, .,,,; 2-3min , . () 10

(3)a,b 7 1 10 1 B , 1 , .

(1) (2)() ,,() (3)a 10 1 a

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,. (1)(),. (2),(),; pH, ;(). ,. (3)10 1 ,. (1), ,.

34.(8 ) Fe3+, Fe3+,, . mRNA , ,. Fe3+, Fe , mRNA , mRNA ,(). :
3+

(1) .



,



(2) Fe ,

3+

,;

Fe3+ , Fe3+ , mRNA .
, . (3) n . mRNA 3n, .

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(4),( UUA,UUG,CUU,CUC,CUA,CUG), DNA , .

(1)GGU, -----GGUGACUGG--------CCACTGACC--

(2) mRNA Fe3+ (3)mRNA (4)CA

, , (1) tRNA CCA GGU,--- ------GGUGACUGG-------------CCACTGACC-----(2) Fe3+,,, mRNA ,, Fe3+,, . (3)mRNA ,. (4) UUG, ACC, C-A , UUG,.


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